关键词: 氯胺酮, LY354740, 老年鼠, 认知障碍

Abstract: 【Abstract】〓Objective〓To evaluate the effects of LY354740 pretreatment on the learning and memory impairment induced by Ketamine in old mice. Methods〓Thirty-six healthy male C57BL/6 mice, aged 18 months, weighing 20-30 g, were randomized into 3 groups (n=12 each), ketamine group (group K), Ly354740 pretreatment group (group L), and control group (group C). In K and L groups, ketamine 30 mg/kg was injected intraperitoneally three times a day at 30-minute intervals for 2 weeks, while in group L, Ly354740 10 mg/kg was injected intraperitoneally at 30 min before the first injection of ketamine, and the group C was injected with the same dose of saline. Cognitive function was assessed using Morris water maze and open field tests after the administration of ketamine was finished. Results〓Compared with controls of group C, the animals in group K showed significantly prolonged escape latency, shortened time spent in the original platform and in the central area for the open field, likewise, the frequency of crossing the original platform was decreased (P<0.05). Compared with group K, the results above-mentioned were significantly meliorated in the Ly354740 pretreatment mice of group L (P<0.05). Conclusion〓Ly354740 pretreatment can attenuate cognitive dysfunction induced by chronic exposure to ketamine in old mice.

Key words: Ketamine, LY354740, Old mice, Cognition disorders