关键词: siRNA, 真核表达载体, OX40, 免疫保护

Abstract: 【Abstract】〓Objective〓To value the immunoprotection of transplant heat that was mediated by eukaryotic expression vector of siRNA technique. Methods〓First, we did mouse heart transplantation using strains representing minor histocompatibility complex antigen mismatches (mHC), and all the experimental animals were divided into three groups. Then we injected respectively sodium chloride, pRNAT-CTL negative control vector and pRNAT-OX40siRNA into the three groups of mouse thymus at the 1st day and the fourth day after heart transplantation..At the 7th day of post-transplantation, we collected the transplant heart,.blood serum and spleen for pathological examination, besides cardiac muscle zymogram,..RT-PCR and Western blot tests,.respectively..Results〓The survival time of transplant heart in pRNAT-OX40siRNA was significantly prolonged,..and the transplant heart pathological examination was significantly bettered and lactate dehydrogenase,.glutamic oxalacetic transaminase, troponin, spleen OX40mRNA expression and OX40 membrane protein expression were significantly lowered compared with those in mice with sodium chloride and pRNAT-CTL negative control vector..Conclusion Our results clearly show that blockade OX40/OX40L pathway has immunoprotection for heart transplantation of mouse with mHC-incompatible.

Key words: siRNA, Eukaryotic expression vector, Immunoprotection