关键词: 时辰化疗, 吉西他滨, 肺癌A549细胞

Abstract:

【Abstract】〓Objective〓To construct the model of nude mice bearing lung cancer A549 cell line. The curative effect and side effect were observed after gemcitabinechrono-chemotherapy, and the best time to administer drug was screened out. Methods〓A549 cells (2×10⁷/mL) in exponential phase of growth were vaccinated in the nude mice by subcutaneous injection. Gemcitabine(50 mg/kg) was administrated by intraperitoneal injection at 2 am,8 am,14 pm in experimental groups and equal sodium chloride in control group when the tumor growing to reach 1 cm×1 cm×1 cm approximately. The growth and weight curves were drawn by measuring data and blood and tissue specimen were hurvested. Results〓Gemcitabine had therapeutic effect on nude mice bearing tumor, in which the lesion of lung, liver and renal in the therapeutic groups was alleviated as compared with that in control group, and hepatorenal function was ameliorated in the administrated group. But there were differences among those groups on the therapeutic efficacy, the 2 am group showed the best effect while the 8 am group had the worst effect(P<0.05, 8 am, 14 pm, 20 pm group vs. hepatoma model group; P<0.01, 2 am group vs. hepatomamodel group; P<0.05, 2 am group vs. 8 am group). Conclusion〓Gemcitabine has therapeutic effect on nude mice bearing tumor, which can retain the growth of tumor, may alleviate the lesion of lung, liver and renal, ameliorate hepatorenal function caused by tumor as well. There were different curative effects in accordance with different administration time. There was the best therapeutic effect in the 2 am group while there was the worst effect in 8 am group.

Key words: Chrono-chemotherapy, Gemcitabine, A549 cell line