关键词: 甲状腺癌, CD8+CD28-调节性T细胞, 细胞因子, 外科手术

Abstract: 【Abstract】〓Objective〓To explore the changes and significance of CD8+CD28- regulatory T lymphocytes for patients with thyroid carcinoma undergoing radical mastectomy. Methods〓Thirty cases with bilateral thyroid carcinoma treated with surgical treatment were selected as the observation group (A group), and 30 cases with benign thyroid tumor and healthy volunteers were served as controls (A group and C group). Using flow cytometry detected three sets of peripheral blood CD8+CD28- regulating T cell growth factors and their intracellular cytokines 1 (TGF-beta 1) and the frequency of interleukin 10 (IL-10). The indexes obove were compared between. Results〓Compared with group C,A and B group of CD8+CD28-T cells and TGF-beta 1 were higher,but no statistical difference was found between group B and C,and the group A were significantly higher than that of group C (P<0.05). Compared with group C,IL-10 in A and B group were significantly increased, and the comparison between two groups have statistical difference(P<0.05). In 4 weeks after surgery in group A, CD8+CD28- T cells,TGF-beta 1 and IL-10 level were reduced than that before the operation (P<0.05), especialy CD8+CD28- T cells and TGF-beta. Conclusion〓CD8+CD28- T cells, TGF-beta 1 and IL-10 expression increases are important immunological factors in patients with thyroid cancer. TGF-beta 1 is relatively specific cytokines and is more sensitine than IL-10 in evaluating the operative efficacy.

Key words: Thyroid carcinoma, CD8+ CD28- regulatory T cells, Cytokines, Surgery