关键词: 姜黄素, LY294002, 前列腺癌, 细胞凋亡

Abstract: 【Abstract】〓Objective〓To explore the effect of curcumin in combined with LY294002 on prostate carcinoma PC-3 cells and the possible mechanism. Methods〓The experiments were divided into four groups according to different drug administration: control group (PPS), curcumin group (25 μmoL/L of curcumin), LY group (25 μmoL/L of LY294002) and combination group (mixture of curcumin and LY294002). Then proliferation viability of PC-3 cells was observed by MTS assay, apoptotic rate was detected by flow cytometry,.the mRNA expression of NF-κB、P53 and caspase-9 were examined by Q-PCR,respectively...Results....PC-3 cell proliferation was inhibited in three groups, curcumin group, LY294002 group and combination group. The proliferation rate in combination group was more decreased than that in other two groups..The apoptosis rate of PC-3 cells in combination group was significantly increased when compared with curcumin group or LY294002 group(P<0.05)..NF-κB expression in combination group was also significant lower than curcumin group or LY294002 group (P<0.05), while the expression of P53 and caspase-9 in combination group were obviously higher than these in groups of drug administrated alone (P<0.05). Conclusion〓The combined use of curcumin and LY294002 can effectively inhibit the growth of PC-3 cells in vitro, its mechanism might be inhibiting cell proliferation by decreasing the expression of NF-κB, and promoting cell apoptosis by increasing the expression of P53 and caspase-9.

Key words: Curcumin, LY294002, Prostate cancer, Apoptosis