关键词: 肝细胞癌, 乙型肝炎病毒, 乙型肝炎病毒X蛋白, 肿瘤病理分级, 无病生存期

Abstract: 【Abstract】 Objective To detect the expression of HBx in HCC and analyze its relationship with disease-free survival (DFS) in HBV-related HCC. To evaluate the detection performance of HBx antigen in human serum. Methods Immunohistochemical staining was utilized to detect the expression of HBx in 35 HBV-related HCC tissues, and results were analyzed with clinical data. Enzyme-linked immunosorbent assay (ELISA) was applied to detect the HBx antigen in human serum. Results HBx positive ratios were 20/35 (57.14%) in HCCs, with 15/24 (62.50%) of HBsAg positive and 5/11 (45.45%) of HBsAg negative cases respectively. The poorly differentiated ratio of HBx positive (9/20, 45.00%) was significantly higher than that of HBx negative (2/15, 13.33%) cases (P<0.01). Disease free survival (DFS) of HBx positive and negative group was estimated by Kaplan-Meier method and compared by log-rank test. The results showed poor DFS in HBx positive group (P=0.03). ELISA could efficiently detect HBx antigen in human serum with 48/77(62.34%) positive. Conclusions The expression of HBx is correlated with tumor pathological grading and poor DFS of HCC patients. ELISA can efficiently detect HBx antigen in human serum.

Key words: Hepatocellular Carcinoma, Hepatitis B Virus, Hepatitis B Virus X Protein, Tumor Pathological Grading, Disease-Free Survival