关键词: 网/腹膜, 乳斑, 巨噬细胞, 表型, 结直肠癌

Abstract: 【Abstract】 Objective To investigate the relationship between the phenotypes of macrophages in omental/peritoneal milky spots and the clinical stages of colorectal carcinomas. Methods Omental and peritoneal specimens of colorectal carcinomas with different stages (60 cases) and normal person (10 cases)were collected in the central hospital of Panyu district from January 2011 to December 2012. After the macrophages had been isolated and purified,the phenotypes of macrophages in omental/peritoneal milky spots were identified and determined by immunofluorescence staining and Flow Cytometry (FCM). And the secretion of IL-10 and IL-12 in different time points were detected by Enzyme linked immunosorbent assay (ELISA). Results The number of M1 type macrophages in control and T1-3 group was larger than that in T4 group (P all<0.01). The number of M2 type macrophages in T4 group was larger than that in control and T1-3 group (P all<0.01). The number of M1 type macrophages was negative correlated with T stages of colorectal carcinomas (P<0.01). But the number of M2 type macrophages was positive correlated with T stages of colorectal carcinomas (P<0.01). There was significant differences in the secretion of IL-10 and IL-12 in different time points among three groups: the IL-10 in control and T1-3 group were lower than T4 group (P all<0.05); the IL-12 in T4 group were lower than control and T1-3 group (P all<0.05). The secretion of IL-12 in macrophages in omental/peritoneal milky spots was negative correlated with T stages of colorectal carcinomas (P<0.01). On the contrary, the secretion of IL-10 was positive correlated with T stages (P<0.01). There was a close correlation between the phenotypes of macrophages in milky spots in the omentum/peritoneum and clinical staging in patients of colorectal carcinomas, especially serosa tumor invasion and tumor lymphnode metastasis (P<0.05). Conclusion The phenotypes of macrophages in milky spots in the omentum/peritoneum is influenced by serosa tumor invasion and tumor lymphnode metastasis; Milky spots mainly express M1 type macrophages in patient with colorectal carcinomas in stage T1-3, it can capture and kill tumor cell; Milky spots mainly express M2 type macrophages in the T4 stages, and promote role for the growth of tumor.

Key words: Omentum/peritoneum, Milky spots, Macrophage, Phenotype, Colorectal carcinoma