关键词: 声诺维超声辐照, 超顺磁性氧化铁纳米粒子, 人类上皮源性肝癌细胞(HEP-G2), 体外成像, 磁共振

Abstract: 【Abstract】 Objective To investigate the feasibility of using SonoVue-enhanced ultrasound exposure (SEUE) to improve the efficiency of SPIO-labeling Hep-G2 cells in vitro. Methods Seven groups of SPIO at different concentration were incubated with Hep-G2 cells,.then processed by SonoVue-enhanced ultrasound exposure (SEUE) for 1 minute. The labeling efficiency, viability and proliferation were examined. The group of 375 μg[Fe]/mL SPIO-labeled Hep-G2 cells which achieved an appropriate level of proliferative activity at cellular densities (cells/mL) of 1×102, 1×103, 1×104, 1×105, 1×106, and 1×107 were processed by an in vitro MRI scan using a clinical 1.5T MRI system. Results A labeling efficiency >90% was achieved in seven experimental subgroups of group A, but they were about 2% in control group B and C. Hep-G2 cells was demonstrated reduced viability as the SPIO concentration increased. The viability of cells in four groups was only 59.7%±7% (500 μg[Fe]/mL), 47.76%±9% (625 μg[Fe]/mL), 46.27%±10% (750 μg[Fe]/mL), and 43.28%±7% (875 μg[Fe]/mL) respectively, which suggests that an SPIO concentration equal to or greater than 500 μg[Fe]/mL is obviously toxic to Hep-G2 cells. The proliferation of SPIO-labeled cells did not differ significantly. The in vitro MRI scanning data showed that the MRI signal intensity of labeled cells decreased with the T2W1 sequences which represents MRI can sensitively detect the SPIO concentration changes in cells. There was an inverse correlation between the labeling cell population and the signal intensity (P<0.05). Conclusions SonoVue-enhanced ultrasound exposure for improving the labeling of Hep-G2 cells with SPIO in vitro is feasible and effective method for MRI scanning.

Key words: SonoVue-enhanced ultrasound exposure (SEUE), Superparamagnetic iron oxide (SPIO), Human epithelial derived hepatoma cells (Hep-G2), In vitro imaging, Magnetic resonance imaging (MRI)